LOCUS       sequence-102608-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pDS-RED-C1-Beclin1
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..735
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(241..261)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     653..676
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc
      121 accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      241 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      301 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg cttcatctac
      421 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca gaagaagacc
      481 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      541 acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
      601 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc caagctggac
      661 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac cgagggccgc
      721 caccacctgt tcctgagatc tcgagctcaa gcttcgaatt ctatggaagg gtctaagacg
      781 tccaacaaca gcaccatgca ggtgagcttc gtgtgccagc gctgcagcca gcccctgaaa
      841 ctggacacga gtttcaagat cctggaccgt gtcaccatcc aggaactcac agctccatta
      901 cttaccacag cccaggcgaa accaggagag acccaggagg aagagactaa ctcaggagag
      961 gagccattta ttgaaactcc tcgccaggat ggtgtctctc gcagattcat ccccc
//
