LOCUS       sequence-102668-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector 
            between HindIII and KpnI sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Tang X, Zhang Y, Tucker L, Ramratnam B
  TITLE     Phosphorylation of the RNase III enzyme Drosha at Serine300 or 
            Serine302 is required for its nuclear localization.
  JOURNAL   Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. 
            Epub 2010 Jun 16.
  PUBMED    20554852
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkq547"; journalName: "Nucleic Acids Res"; date: 
            "2010-10"; volume: "38"; issue: "19"; pages: "6610-9"
COMMENT     Sequence Label: GFP-Drosha
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     250..271
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    493..627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(530..549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     584..603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(650..666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(650..666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(663..685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    674..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(698..704,705..722,723..728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcacntcctg gggcacnagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
       61 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      181 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      301 cgagctgtac aagtaaagcg gccgcgactc taggggatag gtacctcgcg agatctagag
      361 tcgacctgca ggatatcgaa ttcattgatc ataatcagcc ataccacatt tgtagaggtt
      421 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      481 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      541 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      601 atcaatgtat cttatcatgt ctggatccgt accgagctcg cgtaatcatg tcatagctgt
      661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      721 agtgtaaagc ctggggtgcc taatgagtga gctaactaca ttaattgcgt tgcgctcact
      781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      961 cacagaatc
//
