LOCUS       sequence-102674-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express E2 UbcH5B in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, 
            Wolberger C
  TITLE     E2 ubiquitin-conjugating enzymes regulate the deubiquitinating 
            activity of OTUB1.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. 
            Epub 2013 Aug 18.
  PUBMED    23955022
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2655";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1033-9"
COMMENT     Sequence Label: UbcH5B
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..71)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg attaaccctt ttcgaactgc
       61 gggtggctcc aggcctcgat tacattgcat atttctgagt ccattcccga gctattctgt
      121 tgtacttttc tctatctgtt ttgtagatcc gagcaatctc aggcactaaa ggatcatctg
      181 gattgggatc acacaacaga gaacagatgg acaagagtac ttttgaaata gttagtgctg
      241 gagaccactg tgatcgtaga atatcaagac aaatgctgcc attactgtta atatttggat
      301 gataaattct tgttgtaaat gcaaccttag gtggtttgaa ggggtaatct gttgggaaat
      361 gaattgtcaa gaaaaatact ccaccctgat agggactgtc atttggcccc attattgtag
      421 cttgccaatg gaacatatca tctccaacag gacctgctga acactgtgct ggagggtccc
      481 gtgccagatc attcaattcc ttgtggattc tcttcagagc catacctcct cccgtctgct
      541 gttggaacac atcaattgta tcttcatcct ccatttccaa ctgtgcaggt gtgtctgttt
      601 cattgattgg ttgcccgtca aatcggaatc tgatctgcct cattgacaat ccctgtcgtt
      661 cacaataggc tttcattagt ttactaagtg gtgtatgcct cttaatctta aactgcacca
      721 ca
//
