LOCUS       sequence-102677-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cleave the SUMO off when use PETSUMO vector for express fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, 
            Wolberger C
  TITLE     E2 ubiquitin-conjugating enzymes regulate the deubiquitinating 
            activity of OTUB1.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. 
            Epub 2013 Aug 18.
  PUBMED    23955022
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2655";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1033-9"
COMMENT     Sequence Label: SENP2M497A
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    63..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             170..187
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
       61 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
      181 cggcagccat atggatctcc ttgaacttac agaggacatg gaaaaggaaa tcagtaatgc
      241 cctaggccat ggcccacagg atgaaatcct aagtagtgct ttcaaattgc gaattactcg
      301 aggagatatt cagacattaa agaactatca ctggctcaat gatgaagtca ttaattttta
      361 catgaatctt ctggtggaaa gaaataaaaa gcaaggctat ccagcacttc atgtattcag
      421 tactttcttc tatcctaaat taaagtctgg gggttaccaa gcagtgaaac gatggaccaa
      481 aggggtaaat ctctttgaac aagaaattat tctggtgcct attcatcgga aggtacattg
      541 gagcctggtg gtgattgacc taagaaaaaa gtgtcttaaa tatctggatt ctgcgggaca
      601 aaagggccac aggatctgtg agattctcct tcagtattta caggatgaaa gtaagaccaa
      661 aag
//
