LOCUS       sequence-102682-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of NLS-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Zuleger N, Boyle S, Kelly DA, de las Heras JI, Lazou V, Korfali N, 
            Batrakou DG, Randles KN, Morris GE, Harrison DJ, Bickmore WA, 
            Schirmer EC
  TITLE     Specific nuclear envelope transmembrane proteins can promote the 
            location of chromosomes to and from the nuclear periphery.
  JOURNAL   Genome Biol. 2013 Feb 15;14(2):R14. doi: 10.1186/gb-2013-14-2-r14.
  PUBMED    23414781
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2013-02-15"; volume: "14(2):R14. doi"; pages: " 
            10.1186/gb-2013-14-2-r14"
COMMENT     Sequence Label: NLS pEGFP-N3 (1299)
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..58)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(108..311)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(137..157)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        312..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cagaattcga agcttgagct cgagtccccg gatcttccac tttgcgtttt tttttcggcg
       61 gggtccccgc tgtccccatg ctagcggatc tgacggttca ctaaaccagc tctgcttata
      121 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      181 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      241 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      301 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      361 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      421 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      481 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      541 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      601 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
      661 tactattaat aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc
      721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaa
//
