LOCUS       sequence-102711-         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes cytoplasmic cerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-C
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        284..302
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     284..301
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(357..390)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(398..431)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(440..473)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      487..496
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgagcccta cgactctggc tgagtgccac accgccggca gccgcaaagc ggctcaaacc
       61 attgcctttt atggtaataa tgagagaatg cagagggact tcctttgtct ggcatatctg
      121 aggcgcgcgc tgtcactagc gcccaccagc ggtcagactg tagaatgcag agcaaaacag
      181 gaagttgact ccagatggtc acatgcttgc tgaaacncct ctccctggca gcagtgcaat
      241 tctaacagtt tccctttctt ttacagttcg ccatcctagg cctatttagg tgacactata
      301 gaagagtaca agctacttgt tctttttgca ggatccgcca ccatgactag ctcgagataa
      361 cttcgtatag tataccttat acgaagttat cgagctcata acttcgtata ggatacttta
      421 tacgaagtta taactcgaga taacttcgta tagcatacat tatacgaagt tataccggca
      481 gatctcgcca ccatggtgag tgtgattaaa ccagagatga a
//
