LOCUS       sequence-102741-         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for 
            pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring 
            autophagy, based on FUGW (3rd gen lentiviral plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: FUGW-PK-hLC3∆G
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(187..215)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
       61 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggg
      121 ctgcaggtcg actctagagg atccgctagc gccaccatga gtaaaggaga agaacttttc
      181 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      241 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      301 actactggaa aactacctgt tccatggcca acacttgtca ctacgttaac ttatggtgtt
      361 caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
      421 cccgaaggtt acgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      481 cgtgctgaag tcaagtttga aggtgatacc cttgttaat
//
