LOCUS       sequence-102745-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating full length gene 60 mRNA by in vitro 
            transcription with T7 RNA pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(677..693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(690..712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    701..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(725..731,732..749,750..755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    770..791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(908..925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctctagataa tacgactcac tataggagca tttaatgaat ttaaacgcgg ttggattcct
       61 gatgaaaagt tctatgaggt gtataatgaa atttgtaaaa attgattctt ctagcgttga
      121 tatgaaaaaa tataaattgc agaacaatgt tcgtcgttct attaaatcct cttcaatgaa
      181 ctatgcgaat gtcgctatta tgacagacgc agatcacgat ggatagcctt cgggctatct
      241 atagaaatac ctcataatta agagattatt ggattaggtt ctatttatcc ttctctgctc
      301 ggatttttta gtaattggcc agaattgttt gagcaaggac gaattcgctt tgtcaaaact
      361 cctgtaatca tcgctcaggt cggtaaaaaa caagaatggt tttatacagt cgctgaatat
      421 gagagtgcca aagatgctct acctaaacat agcatccgtt atattaaggg acttggctct
      481 ttggaaaaat ctgaatatcg tgagatgatt caaaacccag tatatgatgt tgttaaactt
      541 cctgagaact ggaaagagct ttttgaaatg ctcatgggag ataatgctga ccttcgtaaa
      601 gaatggatga gccagtagtt tactttacca caaggatgtg gtataattaa ttgggcaaaa
      661 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      961 ctcactcaaa ggcggtaata cgg
//
