LOCUS       sequence-102750-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, sequence 5' of coding gap 
            only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 to gap
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(294..310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(294..310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(307..329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    318..334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(342..348,349..366,367..372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    387..408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(525..542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(776..795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccggggatcc tctagataat acgactcact ataggagcat ttaatgaatt taaacgcggt
       61 tggattcctg atgaaaagtt ctatgaggtg tataatgaaa tttgtaaaaa ttgattcttc
      121 tagcgttgat atgaaaaaat ataaattgca gaacaatgtt cgtcgttcta ttaaatcctc
      181 ttcaatgaac tatgcgaatg tcgctattat gacagacgca gatcacgatg gatagtttac
      241 tttaccacaa ggatgtggta taattaattg ggcaaaagct tggcgtaatc atggtcatag
      301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      481 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacg
//
