LOCUS       sequence-102751-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, sequence 5' of coding gap + 
            Cys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 to gap + Cys
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(296..312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(296..312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(309..331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    320..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(344..350,351..368,369..374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    389..410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(527..544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(778..797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggggatcct ctagataata cgactcacta taggagcatt taatgaattt aaacgcggtt
       61 ggattcctga tgaaaagttc tatgaggtgt ataatgaaat ttgtaaaaat tgattcttct
      121 agcgttgata tgaaaaaata taaattgcag aacaatgttc gtcgttctat taaatcctct
      181 tcaatgaact atgcgaatgt cgctattatg acagacgcag atcacgatgg atgttagttt
      241 actttaccac aaggatgtgg tataattaat tgggcaaaag cttggcgtaa tcatggtcat
      301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgg
//
