LOCUS       sequence-102756-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7-transcription template for Tte mRNA used in SiM-KARTS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Rinaldi AJ, Lund PE, Blanco MR, Walter NG
  TITLE     The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs 
            shows ligand-dependent accessibility bursts.
  JOURNAL   Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976.
  PUBMED    26781350
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-01-19"; volume: "7:8976. doi"; pages: " 
            10.1038/ncomms9976"
COMMENT     Sequence Label: pUC19_extendedTTE1564
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggggatcct aatacgactc actatagggg aactcctact acaagttgct aagaggctat
       61 tttttagttc aaattactca tacaatcatg ttaaaattaa tcgcagtgag caacaaaatg
      121 ctcacctggg tcgcagtaac cccagttaac aaaacaaggg aggtaatttt gtgcccaaaa
      181 aaagaataaa agatttagct gaaattgctc ttgttgcagc aatttatttc gcactcacaa
      241 ttatattttc gtccatttcg tttttacccg ttcaatttcg aatcggggaa attacgaaat
      301 ccattgtagt attcaataaa aaatatgcta tttccatgat gataggaaat ttttttgcaa
      361 atttgtttag cccatttgct ggtgcaatgg aattaatttt tatgcctctt tcgaacttaa
      421 taggctgtac aattggatac tacattggaa gacttactca caaagcgata ggagctatat
      481 tcatagccct ttggattgca gcatcagttg caattacttt aaaggtttct gcaggcatac
      541 catttattcc gactttctta agcgtgggag tagcggaaac tgtacttttg gtaactggat
      601 attttttgct tttcacaatt gaaaagaaag gagttgtgaa atttgacaga taaatataaa
      661 gagagaagat ttgacattta cggttacgaa aaaattgaca aagaagttct agaatctatt
      721 gaatatgagt atcctgaaaa aaatactatc gtggagtata ttaccgatga attttcttct
      781 gtttgccctt ggacaggatt acctgacaat gcaaaactta ctataaggta tataccc
//
