LOCUS       sequence-102759-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For use in trypanosome cell lines already expressing T7 polymerase 
            and Tet-repressor. Tetracycline induction results in the production 
            of dsRNA targeting VSG221..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Sheader K, Vaughan S, Minchin J, Hughes K, Gull K, Rudenko G
  TITLE     Variant surface glycoprotein RNA interference triggers a 
            precytokinesis cell cycle arrest in African trypanosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8716-21. Epub 2005 Jun
            3.
  PUBMED    15937117
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-06-14"; volume: "102(24)"; pages: 
            "8716-21. Epub 2005 Jun 3"
COMMENT     Sequence Label: MC177VSG221RNAi
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(547..594)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     590..608
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac
       61 ggcactggtc aacttggcca tggtgatagc ttattttatg gcagcaacga gaccttacgt
      121 aaagcaaaac taaataagag cggagactgc aatgcagagt aaaaaaaaaa aaaacatgaa
      181 tttcagaaga ccttgctgtg cccgggtctg agagcggtca gttgctctcc tgctccgtac
      241 gccgtaagcg ctacttttac tgcttaatct ttcctatata agatatattt ctattactat
      301 atatactttt ttaactttat cattattatc ctattgtgtg ttgtattgac tttacaataa
      361 aacatattat atctaacatg tcaaaagcat cggctcttca atacagttac ccgtccctta
      421 tcaacacagt acaatatact ctgtcatgac aataagtata tataagagcc agaatgcacc
      481 cgcgctgggt ggaaagctag cttgcatgcc tgcaggggcc caatccggat atagttctcc
      541 tttcagcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg
      601 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccctatccgg
      661 atatagttct cctttcagc
//
