LOCUS       sequence-102760-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For use in trypanosome cell lines already expressing T7 polymerase 
            and Tet-repressor. Tetracycline induction results in the production 
            of dsRNA targeting VSG221..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Sheader K, Vaughan S, Minchin J, Hughes K, Gull K, Rudenko G
  TITLE     Variant surface glycoprotein RNA interference triggers a 
            precytokinesis cell cycle arrest in African trypanosomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8716-21. Epub 2005 Jun
            3.
  PUBMED    15937117
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-06-14"; volume: "102(24)"; pages: 
            "8716-21. Epub 2005 Jun 3"
COMMENT     Sequence Label: MC177VSG221RNAi
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     361..379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(417..439)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     539..558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     768..787
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      361 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
      421 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      481 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      541 gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg
      601 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
      661 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
      721 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
      781 atataggcgc cagc
//
