LOCUS       sequence-102761-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7-transcription template for CAT control mRNA used for in vitro 
            translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Rinaldi AJ, Lund PE, Blanco MR, Walter NG
  TITLE     The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs 
            shows ligand-dependent accessibility bursts.
  JOURNAL   Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976.
  PUBMED    26781350
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-01-19"; volume: "7:8976. doi"; pages: " 
            10.1038/ncomms9976"
COMMENT     Sequence Label: pAMB_CAT-FspI
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..673
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(83..102)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             674..691
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(811..829)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 agaaggagat atacatatgg agaaaaaaat cactggatat accaccgttg atatatccca
       61 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      121 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      181 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
      241 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
      301 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
      361 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      421 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      481 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      541 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      601 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      661 gcagggcggg gcgcatcatc atcatcatca ttaaggatcc gaattcgagc tccgtcgaca
      721 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taacc
//
