LOCUS       sequence-102763-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts a puromycin resistance gene and a VSG117 gene into the 
            VSG221 expression site of T.brucei downstream of the expression site
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Smith TK, Vasileva N, Gluenz E, Terry S, Portman N, Kramer S, 
            Carrington M, Michaeli S, Gull K, Rudenko G
  TITLE     Blocking variant surface glycoprotein synthesis in Trypanosoma 
            brucei triggers a general arrest in translation initiation.
  JOURNAL   PLoS One. 2009 Oct 26;4(10):e7532. doi: 
            10.1371/journal.pone.0007532.
  PUBMED    19855834
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-26"; volume: "4(10):e7532. doi"; pages: " 
            10.1371/journal.pone.0007532"
COMMENT     Sequence Label: p221- purVSG117UTR
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctggagctc caccgcggtg gcggccgctc tagaactagt gggaaatatt tatttgttat
       61 tttttctttt attagctggt tcttagggtg atttatgttt ccggattaaa ctaacaagct
      121 gatagtttgg tttacttatt gtctttctgt ggggaggaat gttggtgatt tgttcggaga
      181 atatttcgga tgcttttttt ttactagatg aggacatcaa ctcgccaatt gtggatggga
      241 gtttgttggc tgaatagtat tatggatcag gtcagaggca tgtttttcag tgttacggac
      301 cgcgtgtgga tgaaacacta gaatgtgttg ccttttgtct tgagtatgaa tttggccgta
      361 acgattgttg ttgcagtatg tttttcttca acaaaaaaaa gtttcaatga aatttgtaat
      421 ccactttaat acggttacaa ctttcgcatt tacaactgat gctatatcga tctgacgact
      481 aaccttgatg ttaaataggt aagtttttac agatgctagg ctccaattta tagtgaagta
      541 agcaatcgta aagttggtga ttcttccttt atgggtatga ggtaaggaat atcgacgtgc
      601 atatcactaa agccttcttt agtacttttc acagggttag aatcactata atgctgagcg
      661 atatttcggt tttttactaa gaaaagacac tggataatac atttgtcgca gc
//
