LOCUS       sequence-102764-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inserts a puromycin resistance gene and a VSG117 gene into the 
            VSG221 expression site of T.brucei downstream of the expression site
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Smith TK, Vasileva N, Gluenz E, Terry S, Portman N, Kramer S, 
            Carrington M, Michaeli S, Gull K, Rudenko G
  TITLE     Blocking variant surface glycoprotein synthesis in Trypanosoma 
            brucei triggers a general arrest in translation initiation.
  JOURNAL   PLoS One. 2009 Oct 26;4(10):e7532. doi: 
            10.1371/journal.pone.0007532.
  PUBMED    19855834
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-10-26"; volume: "4(10):e7532. doi"; pages: " 
            10.1371/journal.pone.0007532"
COMMENT     Sequence Label: p221- purVSG117UTR
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     441..460
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
       61 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
      121 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
      181 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
      241 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
      301 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
      361 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
      421 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggaaggtcgt ctccttgtga
      481 ggggtcaggg gcgtgggtca ggggatggtg gcggcaccgg tcgtggcggc catcaagcta
      541 tcgaattaat tcctgcagaa gtgctttgtt gttgttgtta gtggtgctag tacctactgt
      601 tgctaatata aacggatgaa tagtcctttg cttaaactag tgtttgaaga tctgtcagaa
      661 atcagcaccg cgagggggtg aggagaggag aacatagagt caacattagg caatacttac
      721 gccaccacca cttacgaagt gagaagtgcg tgtaggcgca ctccacacag cggaaga
//
