LOCUS       sequence-102768-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For replacement of VSG221 with a blasticidin resistence gene in the 
            VSG221 expression site in T.brucei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     pBSVSG221KOblast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBSVSG221KOblast
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctggagctc caccgcggtg gcggccgctc tagatgcagc atatgtaact acctctcgtc
       61 acaggcagac gcaagccaaa cagctgatga ttgcaccacg tcttgcagaa actgttaaaa
      121 gcagtgccaa tttagaaaga ataaaaggaa atgagagaat gtacaaataa aggaaataaa
      181 aagaaaaact tttattgcac cttaggatag tgtggaaacg tttaacatta atggagcgta
      241 cacacaagtg aattttctaa actattagat ggcgctcagg tttttctgat tatctaaaat
      301 gaaggcagcg gaaagtgtgc caatgcattt taaatgacag atttatttta ttaaaaatga
      361 gaacacttag aatatttcat gtcgattcaa gctagcagta atatgccagt aaatagtttc
      421 catttacaag cacaatttca tcctccttac gtgattctgt agcatttagt tcaagaaaaa
      481 gcaataaaag aattcggaga cgaagagccg gttagattgc atttggacac cgctatacat
      541 atgttaagac acacaagcat tctatacgta aaagatctag tatataggag caacgctctg
      601 ccaaaacata atggcaagac aaacggccgt gtttgccgct gatgctacag aaccagctta
      661 atttccagaa gacgaaaatt tgcatgtttt cccacaatat tttaattact cttgaagatt
      721 gtagttattc ctacgcgaca ggatcccccg ggctgcagga attcgataat tcatggccaa
      781 g
//
