LOCUS       sequence-102783-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        276..294
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     276..293
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             319..499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    519..552
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             802..813
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg
       61 accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag
      121 aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc
      181 ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac
      241 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaatacac
      301 ggaattaatt ctagatgctg ctagagattt tccacactga ctaaaagggt ctgagggatc
      361 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
      421 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
      481 ggtctaacca gagagaccca gtacaagcaa aaagcagcat aacttcgtat aatgtatgct
      541 atacgaagtt attcccatgg tgaattgatc ccatcttgtc ttcgttggga gtgaattagc
      601 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
      661 attggtctta aaggtaccga gctcgaattc ccgatgcggg gaggcggccc aaagggagat
      721 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
      781 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
      841 cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa
      901 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
      961 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
     1021 acaggtggtg gcaatgcccc aaccagtggg
//
