LOCUS       sequence-102786-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a gRNA plasmid targeting the prion gene's exon 3, positive
            strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H,
            Schmitt-Ulms G
  TITLE     CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on 
            the Proteome.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114594. doi: 
            10.1371/journal.pone.0114594. eCollection 2014.
  PUBMED    25490046
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114594. doi"; pages: " 
            10.1371/journal.pone.0114594. eCollection 2014"
COMMENT     Sequence Label: MLM3636-Prnp-CDS-Pos
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..125
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        204..279
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(463..482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
       61 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      121 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      181 accggtggaa caccggtgga agcgttttag agctagaaat agcaagttaa aataaggcta
      241 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttaagc ttgggccgct
      301 cgaggtacct ctctacatat gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      361 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      541 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      721 cgccactggc agcagccact ggtaacagga ttagcagagc ga
//
