LOCUS       sequence-102788-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a gRNA plasmid targeting the prion gene's exon 3, negative
            strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H,
            Schmitt-Ulms G
  TITLE     CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on 
            the Proteome.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114594. doi: 
            10.1371/journal.pone.0114594. eCollection 2014.
  PUBMED    25490046
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114594. doi"; pages: " 
            10.1371/journal.pone.0114594. eCollection 2014"
COMMENT     Sequence Label: MLM3636-Prnp-CDS-Neg
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(465..484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccttggc cccatccacc gccatgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa gcttgggccg
      301 ctcgaggtac ctctctacat atgacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg ta
//
