LOCUS       sequence-102789-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a gRNA plasmid targeting the prion gene's exon 3, negative
            strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H,
            Schmitt-Ulms G
  TITLE     CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on 
            the Proteome.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114594. doi: 
            10.1371/journal.pone.0114594. eCollection 2014.
  PUBMED    25490046
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114594. doi"; pages: " 
            10.1371/journal.pone.0114594. eCollection 2014"
COMMENT     Sequence Label: MLM3636-Prnp-CDS-Neg
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(22..270)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(80..99)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(250..270)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        377..481
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(700..719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catggcggtg gatggggcca aggtgtttcg tcctttccac aagatatata aagccaagaa
       61 atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa
      121 aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt
      181 tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa
      241 atatgaagga atcatgggaa ataggccctc ttcctgcccg accttggtac cggatccagt
      301 cgactgaatt ggttccttta aagcctgctt ttttgtacag ctagcgacgt caggtggcac
      361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      721 ccgtattgac g
//
