LOCUS       sequence-102790-         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2,
            Klf4 and Myc for iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: TetO-FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(350..403)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cctggtgggt tagcgagttg gaaaggataa agtctaggtc caggaggtcg ttgaactcct
       61 cggtctccct ccgggctagg agggccgggt tgttactgct gcaagctgca ccagctccgc
      121 cactctccag gtctgtggcc accgtcgccg ccaggtcgta ggggcggccg ggaagtgggg
      181 gaagtcgctt catgtgagag agttcctcac gccaacggtt agtcggggca cctgctggac
      241 gcagtgtctt ctcccttccc gccgggccgg acgcgaacgt ggagaaggac gggagcagag
      301 cgtcgctgac agccatgtca gactcgccag gtggctgcct catctcgagt gggccgggat
      361 tttcctccac gtccccgcat gttagaagac ttcccctgcc ctcgccggag ccgcatgcca
      421 tgtgcgacag gggcagtgtg ccgttaatgg ccgtgccggg caccg
//
