LOCUS       sequence-102795-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the yeast Cwc25 protein for overexpression and purification 
            using a C-terminal hexahistidine tag. A single cysteine residue is 
            encoded after the Cwc25 sequence for fluorescent labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter 
            NG
  TITLE     Biased Brownian ratcheting leads to pre-mRNA remodeling and capture 
            prior to first-step splicing.
  JOURNAL   Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704.
            Epub 2013 Nov 17.
  PUBMED    24240612
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2704";
            journalName: "Nat Struct Mol Biol"; date: "2013-12"; volume: "20"; 
            issue: "12"; pages: "1450-7"
COMMENT     Sequence Label: Cwc25-pRSETA
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             572..592
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             593..610
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(676..694)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      690..737
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agaaggagat atacatatgg ggtcgggcga tttgaatctg ttgaaatcat ggaatcccaa
       61 gcttatgaaa aatagaaaaa aagtatggga gaccgaacag gatctaatta ccgaacagca
      121 gaagcttaac acgcgattga aagaaattga aaaagaacga gaactaaatg aactgctgaa
      181 cgaatcgagc aaggataagc cggagactct caagaatgac ttggctttga agaagtcggg
      241 cttggaatgg atgtatcagg atgcaaaact gagtgacgaa aaggaagact atttattagg
      301 taagaaaaaa ctagattcat ctatactaaa ccaacctgca acaccgcctg taagggcggc
      361 cactactatt tccgcttctg gtgccgcaac atccataagt tcgcagaaga agaagtcaaa
      421 attgcttaaa gatgatccaa tgagtaagtt taaggtaact aaacaacaaa gaagaactcc
      481 agactcaaca aaaaaaagag caatgtcgca acgagggaaa ccgttgtcca agcccgctcc
      541 ggacctagac tacggcgccg gaggcggctg tgaaaatctt tattttcaag gtcatcatca
      601 tcatcatcat taggaattcg aagcttgatc cggctgctaa caaagcccga aaggaagctg
      661 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      721 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atctggcgta atagcgaaga
      781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
//
