LOCUS       sequence-102805-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with
            the TEE and trigger factor sequences inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     Brian Smith Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNIC28-Bsa4-TF
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..170
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata tacatatgaa tcacaaagtg atgcaagttt
      181 cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct gctgacagca
      241 tcgagacggc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt attgacggct
      301 tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc
      361 aggacgttct gggtgacctg atgagccgta acttcattga cgccatcatt aaagaaaaaa
      421 tcaatccggc tggcgcaccg acttatgttc cgggcgaata caagctgggt gaagacttca
      481 cttactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg gaagcgatcg
      541 aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt tgacggcatg ctggatactc
      601 tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg
      661 taaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg
      721 atttcgtact ggcgatgggc cagggtcgta tgatcccggg ctttgaagac ggtatcaaag
      781 gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagaa taccacgcag
      841 aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg
      901 aactgccgga actgactgc
//
