LOCUS       sequence-102810-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with
            the TEE and trigger factor sequences inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Brian Smith Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNIC28-Bsa4-TF
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgt atccaccttt actggagacc
      121 gtcaatgcca ataggatatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg
      181 tccttgttca aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag
      241 gaagctaggc gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct
      301 tgtaatcacg acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt
      361 tacatcgtta ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta
      421 tgggccagtt aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc
      481 gtcaatcgtc atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt
      541 aaagacgttc gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat
      601 cacttttttc agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa
      661 ctcagccgtg cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga
      721 tgtgcttttg ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc
      781 agttccagtg tttgcttcaa atactaagta tttgtggcct ttatcttcta cgtagtgagg
      841 atctctcagc gtatggttgt cgcctgagct gtagttgcct tcatcgatga actgctgtac
      901 attttgatac gtttt
//
