LOCUS       sequence-102832-        6370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Glucarate biosensor. Low copy plasmid that expresses GFP 
            proportional to glucarate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-cdaR
FEATURES             Location/Qualifiers
     source          1..6370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(59..127,128..919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(277..296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1072..2022)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2070..2292
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(2784..2801)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2954..3040
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6289..6336
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgaac ctcagatcct
     1081 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     1141 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
     1201 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
     1261 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     1321 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     1381 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     1441 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     1501 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     1561 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     1621 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     1681 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     1741 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     1801 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     1861 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     1921 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     1981 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     2041 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     2101 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     2161 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     2221 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     2281 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     2341 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     2401 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     2461 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     2521 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     2581 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     2641 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccttgac
     2701 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac tggctgtttt
     2761 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     2821 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     2881 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     2941 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     3001 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     3061 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     3121 tcaaattaag cagaaggcca tccgtcagga tggccttttt gcgtttctac aaactatgct
     3181 gttgattgac gccagtgaga acccggaacc ggaaacggaa tcaaatccgt gggtcgaaca
     3241 gtggggcacg ctgttgtcct gatatgttca gcgagcggta aatgtcgttt tagcggtgct
     3301 gaatcgaatc ttttcaggca aatgccagta aaaactgctt catagcgcgg atttttactg
     3361 gcgtttgcct ggagtcaagc gatccatttc atactcttct ttatttcttc gttttaaccc
     3421 ttcctttctt gttcttgttt tcatttccgt gaagtggatt ccaccgtcca gggctaatgc
     3481 caaaatcggg cctcattgaa cgcattaatg ttgtgttgtt gcacggtgag ccgctatggc
     3541 gcgcttttta tactgctatt gccagatata aacacgcgcc gtattcggcg aacgacctat
     3601 aaaaacggca aaaaacaccc tacgtcacct ctgatttcct ggcgatgtcg cagtccagag
     3661 tgagcgtggc taacgcgaat tttcaggagt gcaacattta actttaagaa ggagatatac
     3721 atatgcgtat aggtgaagaa ctgttcaccg gtgttgttcc gatcctggtt gaactggacg
     3781 gtgacgttaa cggtcacaaa ttctctgttc gtggtgaagg tgaaggtgac gctaccaacg
     3841 gtaaactgac cctgaaattc atctgcacca ccggtaaact gccggttccg tggccgaccc
     3901 tggttaccac cctgacctac ggtgttcagt gcttcgctcg ttacccggac cacatgaaac
     3961 agcacgactt cttcaaatct gctatgccgg aaggttacgt tcaggaacgt accatctctt
     4021 tcaaagacga cggtacctac aaaacccgtg ctgaagttaa attcgaaggt gacaccctgg
     4081 ttaaccgtat cgaactgaaa ggtatcgact tcaaagaaga cggtaacatc ctgggtcaca
     4141 aactggaata caacttcaac tctcacaacg tttacatcac cgctgacaaa cagaaaaacg
     4201 gtatcaaagc taacttcaaa atccgtcaca acgttgaaga cggttctgtt cagctggctg
     4261 accactacca gcagaacacc ccgatcggtg acggtccggt tctgctgccg gacaaccact
     4321 acctgtctac ccagtctgtt ctgtctaaag acccgaacga aaaacgtgac cacatggttc
     4381 tgctggaatt cgttaccgct gctggtatca cccacggtat ggacgaactg tacaaagatg
     4441 catgccagtt ctagcataac cctaatgagt gagctaactt acattaattg cgttgcgcct
     4501 taattaacgg cactcctcag caaatataat gaccctcttg ataacccaag agggcatttt
     4561 ttaatgccca tggcgtttac caaactgacc ctgggcttac tgcgcgacgg taagcaggtt
     4621 aacgtgaacc tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc
     4681 aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac
     4741 aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt
     4801 ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa
     4861 ccgtctgtgc tggcactcaa cattcagcgc ggcgacagca ccatctacct gttaatgcag
     4921 taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt
     4981 caccacaact ccatacatct tcatcatcct ttaggcattt gcacaatgcc gtacgttacg
     5041 tacttcctta tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac
     5101 caaaatggcg caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt
     5161 aatggatgcc cgtgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca
     5221 cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtagc
     5281 acgtcatctg cacggtgtgc ggcaggggat taatctaccg ttacggctgg aaggtgaaat
     5341 tgtcggcgta attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt
     5401 ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg atgcacttgt tggcgcagga
     5461 tagccgtttg cgggaagaac tggtgatgaa cctgattcag gcagaggaga atactcccgc
     5521 acttactgaa tgggcgcaac ggctggggat cgatctcaat caaccgcgag tggtggctat
     5581 tgttgaggtc gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact
     5641 gcaaaacgcg ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctaaccga
     5701 aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg
     5761 taagcgagtt gaacaactga ttacccgcat gaaagagtac ggccagctgc gttttcgcgt
     5821 ttcactgggc aactatttta ccggtcctag cagtattgcc cgatcctatc gtacggcgaa
     5881 aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga
     5941 tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca acgaactggc
     6001 gcgaccgctg gcgcggctga aaacgatgga caataacggc ttgctgcgac gaacgctggc
     6061 ggcgtggttt cgccacaatg tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg
     6121 taataccctg gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggcaa
     6181 ttttgatgac aggttgctgc tgtatgtggc gttacaactg gatgaagagc ggtagtaagc
     6241 gactaaaaaa ttgaatgtag gaaaccaaca tgccagttcg agcaataact agcataaccc
     6301 cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc gagaagcaca
     6361 cggtcacact
//
