LOCUS       sequence-102835-        4897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose biosensor. High copy plasmid that expresses GFP 
            proportional to arabinose concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-araC
FEATURES             Location/Qualifiers
     source          1..4897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(59..127,128..919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(277..296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1090..1678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1579..1598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1767..1784)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1937..2023
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2181..2464
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2527..3240
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(3574..3858)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3885..4763
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      4817..4864
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatccgtca ggatggcctt tttgcgtttc tacaaactcc
     2161 tgctagcaag taaggccgac agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     2221 tgcgtctttt actggctctt ctcgctaacc caaccggtaa ccccgcttat taaaagcatt
     2281 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     2341 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     2401 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     2461 ccatacccgc tttcatatct ttcacttttt ttgggctaac tttaacttta agaaggagat
     2521 atacatatgc gtaaaggtga agaactgttc accggtgttg ttccgatcct ggttgaactg
     2581 gacggtgacg ttaacggtca caaattctct gttcgtggtg aaggtgaagg tgacgctacc
     2641 aacggtaaac tgaccctgaa attcatctgc accaccggta aactgccggt tccgtggccg
     2701 accctggtta ccaccctgac ctacggtgtt cagtgcttcg ctcgttaccc ggaccacatg
     2761 aaacagcacg acttcttcaa atctgctatg ccggaaggtt acgttcagga acgtaccatc
     2821 tctttcaaag acgacggtac ctacaaaacc cgtgctgaag ttaaattcga aggtgacacc
     2881 ctggttaacc gtatcgaact gaaaggtatc gacttcaaag aagacggtaa catcctgggt
     2941 cacaaactgg aatacaactt caactctcac aacgtttaca tcaccgctga caaacagaaa
     3001 aacggtatca aagctaactt caaaatccgt cacaacgttg aagacggttc tgttcagctg
     3061 gctgaccact accagcagaa caccccgatc ggtgacggtc cggttctgct gccggacaac
     3121 cactacctgt ctacccagtc tgttctgtct aaagacccga acgaaaaacg tgaccacatg
     3181 gttctgctgg aattcgttac cgctgctggt atcacccacg gtatggacga actgtacaaa
     3241 gatacatgcc agttctagca taaccctaat gagtgagcta acttacatta attgcgttgc
     3301 gccttaatta acggcactcc tcagcaaata taatgaccct cttgataacc caagagggca
     3361 ttttttaatg cccatggcgt ttaccggatg atgacggaac tggttattcg gggcatcaca
     3421 aaattgccct ttctgccaac ggggatacca ctctacgctg gtggcgatct cttcaccggt
     3481 agcgcagtcc accgccaaag ctcgcacaga atcactgcca aaatcgaggc caattgcaat
     3541 cgccatcgtt tcactccatc caaaaaaacg ggtatggaga aacagtagag agttgcgata
     3601 aaaagcgtca ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg
     3661 tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac
     3721 gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta
     3781 ccggttgggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat
     3841 atggacaatt ggtttcttct ctgaatggtg ggagtatgaa aagtatggct gaagcgcaaa
     3901 atgatcccct gctgccggga tactcgttta acgcccatct ggtggcgggt ttaacgccga
     3961 ttgaggccaa cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata
     4021 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgtct
     4081 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc
     4141 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg
     4201 aatggcttaa ctggccgtca atatttgcca atacgggttt ctttcgcccg gatgaagcgc
     4261 accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc
     4321 gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag
     4381 cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca
     4441 tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct
     4501 tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct
     4561 ggcgcgagga ccaacgcatt agtcaggcga agctgctttt gagcactacc cggatgccta
     4621 tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg cgagtattta
     4681 aaaaatgcac cggggccagc ccgagcgagt ttcgtgccgg ttgtgaagaa aaagtgaatg
     4741 atgtagccgt caagttgtca taataagcga ctaaaaaatt gaatgtagga aaccaacatg
     4801 ccagttcgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     4861 tttgctgaac ctcaggcgag aagcacacgg tcacact
//
