LOCUS       sequence-10285-e        4981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD
  TITLE     Odor coding by a Mammalian receptor repertoire.
  JOURNAL   Sci Signal. 2009  . 2(60):ra9.
  PUBMED    19261596
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Signal.
            2009  . 2(60):ra9."
COMMENT     Sequence Label: pCI-MOR271-1
FEATURES             Location/Qualifiers
     source          1..4981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2011..2019
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2095..2216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2106..2125)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2160..2179
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2397..2852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2484..2503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2694..2715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2919..2938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3038..3060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3098..3116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3184..3288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3289..3357,3358..4149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3507..3526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4320..4908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4809..4828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcatg aatggcacag aaggccctaa
     1081 cttctacgtg cccttctcca atgcgacggg tgtggtacgc gaattcacgc gtatggagaa
     1141 atggaatcag agttcaagtg atttcattct cttagggttg cttccacaaa accaaactgg
     1201 cctactactt atgatgctca tcatacttgt cttctttctg gccttgtttg gaaactcagc
     1261 aatgatccac ctcattcgtg tggatccaag gctccacacc cccatgtact ttctcctcag
     1321 tcagctctct ctcatggacc tgatgtacat ttctaccact gttcccaaga tggcatttaa
     1381 cttcctttct ggccagaaaa acatctcttt tctgggctgt ggtgtgcagt cttttttctt
     1441 tttgaccatg gcaggttctg agggcttgct cttggcttcc atggcttatg atcgttttgt
     1501 ggctatctgc catccccttc actatcccat tcgcatgagc aagataatgt gtctgaagat
     1561 gatcatagga tcctggatat tgggctcaat caactcttta gcacattcca tctatgccct
     1621 tcatattcct tactgccatt ctaggtccat taaccatttc ttctgtgatg ttccagccat
     1681 gttgcccctg gcctgtatgg acacttgggt ttatgagtac atggtgtttg tgagcacaag
     1741 cctgtttctc ctactgcctt tccttggtat cacagcttcc tatggtaggg tcctttttgc
     1801 tgtcttccac atgcgctcaa aagagggaaa gaagaaggcc ttcaccacat gctcaactca
     1861 cttaactgtg gtgacatttt actatgcacc ttttgtctat acctatcttc gacctaggag
     1921 tcttcgctcc ccaacagaag ataagattct gactgttttc tacactatcc ttacccccat
     1981 gctcaacccc atcatctata gtctgaggaa taaggaggtc ctgggggcca tgacaagagt
     2041 ccttgggaca ttctcttcca tgaaaccatg agcggccgct tcgagcagac atgataagat
     2101 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2161 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2221 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     2281 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgggctgg cgtaatagcg
     2341 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
     2401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2521 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2581 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2641 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2701 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2761 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2821 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     2881 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     2941 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3061 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4441 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc
     4981 t
//
