LOCUS       sequence-102877-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of mCherry, EGFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ
  TITLE     PLIC proteins or ubiquilins regulate autophagy-dependent cell 
            survival during nutrient starvation.
  JOURNAL   EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 
            2009 Jan 16.
  PUBMED    19148225
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2008.238"; journalName: "EMBO Rep"; date: "2009-02"; 
            volume: "10"; issue: "2"; pages: "173-9"
COMMENT     Sequence Label: pBABE-puro mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(142..558)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(158..174)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(190..212)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    759..958
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
       61 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc
      121 accatggtgg cagatccggc cggcgcctag agaaggagtg agggctggat aaagggagga
      181 tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg
      241 aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc
      301 ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac
      361 cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac
      421 gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac
      481 cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg
      541 agtggtaaca gtctggccct aattttcaga caaatacaga aacacagtca gacagagaca
      601 acacagaacg atgctgcagc gctgcagcag acaagacgcg cggcttcggt tccaaaccga
      661 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
      721 atatcctgac ggggtcggat tccacatcga ctcccttcct caggtcgggc cacaaaaacg
      781 gcccccaaag tccctgggac gtctcccagg gttgcggccg ggtgttcaga actcgtcagt
      841 tccaccacgg gtccgccaga tacagagcta gttagctaac tagtaccgac gcaggcgcat
      901 aaaatcagtc atagacacta gacaatcgga cagacacaga taagttgctg gccagcttgc
      961 ctcccggtgg tgggtcggtg gtccctgggc aggggtctcc cgatcccgga cgagccccca
     1021 aatgaaagac ccccgctgac gggtagtcaa tcactcagag gagaccctcc caaggaacag
     1081 cgagacc
//
