LOCUS       sequence-102879-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of mCherry, EGFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ
  TITLE     PLIC proteins or ubiquilins regulate autophagy-dependent cell 
            survival during nutrient starvation.
  JOURNAL   EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 
            2009 Jan 16.
  PUBMED    19148225
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2008.238"; journalName: "EMBO Rep"; date: "2009-02"; 
            volume: "10"; issue: "2"; pages: "173-9"
COMMENT     Sequence Label: pBABE-puro mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    266..290
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      299..308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(305..307,308..310,311..1021)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(305..307,308..310,311..1021)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(350..371)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(611..630)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     958..979
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
       61 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
      121 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
      181 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc
      241 cggactcaga tccgctggcc caggaacaag tttgtacaaa aaagcaggct ttccggtcgc
      301 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      361 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      421 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      481 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      541 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      601 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      661 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      721 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      781 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      841 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      901 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      961 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1021 gtccggactc agatccggta ccgaattcat gccgtcg
//
