LOCUS       sequence-10288-e        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Saito H, Chi Q, Zhuang H, Matsunami H, Mainland JD
  TITLE     Odor coding by a Mammalian receptor repertoire.
  JOURNAL   Sci Signal. 2009  . 2(60):ra9.
  PUBMED    19261596
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Signal.
            2009  . 2(60):ra9."
COMMENT     Sequence Label: pCI-MOR277-1
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2110..2231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2121..2140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2175..2194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2412..2867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2499..2518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2709..2730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2934..2953)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3053..3075
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3113..3131)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3199..3303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3304..3372,3373..4164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3522..3541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4335..4923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4824..4843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcatg aatggcacag aaggccctaa
     1081 cttctacgtg cccttctcca atgcgacggg tgtggtacgc gaattcacgc gtatggcctg
     1141 ggcaggcaac cagactctca tctctcactt tgtcctcctg ggcctcttca cccactctcc
     1201 actgcatctc ttcctcttct ccatcattat ggtcatgttc ctggtggccc tctctggcaa
     1261 tgggctcatg atcctcctca tccttatgga ctctcgcctg cacaccccga tgtacttctt
     1321 cctcagttgg ctgtccctca tggacctcat gctcatctcc accattgtgc cacggatggc
     1381 tgctgacttt ctcctgggcc gtggctccat ctccttcgct ggctgtgggc tccagattct
     1441 cttcttcctc accctcctgg gggatgagtg cttcctgcta gccttcatgg cctatgaccg
     1501 ctatgtggcc attagcaacc cgctgagata ctctgtaatc atgagccgcc gtgtctgctg
     1561 gctcatggta gcggggtctt ggctctttgg cctggtagat gggctgatcc aggctgtttt
     1621 tacacttcgc ttcccctact gcggttccca ggagatcgac cacttcttct gtgaggttcc
     1681 tgctgtgctc aagctggcct gtgctgacac cagtctctac gagaccatga tctatgtctg
     1741 ctgtgtcctc atgcttctcc tgcccttttc tgtcatctcg gcttcctacc tcaggatcct
     1801 ggtggcagtg ctccggatgc gctctgcaga agggcggcgg aaggccttcg ctacctgttc
     1861 ctcccacatg atcgtggtct ccctcttcta cggggctgcc atgatcacat acatgaggcc
     1921 acaggcctac cactcctcca agcaggacaa agtggtctct gctttctaca ccatgatcac
     1981 gcccatgctc aaccctctta tttacagtct aaggaacaaa gaagtgaccg gagccctgag
     2041 gaaactactg ggaaagtgtc cctgtggtgg tgggactctt ggttgagcgg ccgcttcgag
     2101 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2161 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2221 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     2281 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg
     2341 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2401 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2821 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     2881 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     2941 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3001 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3061 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3121 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3181 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3241 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3301 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3361 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3421 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3481 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3541 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3601 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3661 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3721 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3781 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3841 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3901 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3961 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4021 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4081 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4141 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4201 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4441 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4561 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4621 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4681 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4741 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4801 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4861 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4921 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4981 atggctcgac agatct
//
