LOCUS       sequence-102883-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human ALG13 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-ALG13 (OTU, aa 216-353)
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(470..493)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(532..554)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgtttaaatg gtctagaaag ctttattgtt
       61 ttgagtacac agaatcatag tgaccactac ttgagtagca gagtagaatc ttgtcttcat
      121 agccattatc tgtgacataa gttggaggtt ttccaggaaa gcgataaaga atgaaatccc
      181 gattataaat tagagaaaga gctcttattt ccagctggcc agcactttcc ttgggatctc
      241 ccaaccgttc caggtatttc tcaaaagatc cctccacata agactcaaaa gtttgttgat
      301 tttccctcat atatgagaca caagccttcc tgatttccaa atgatggacc tggctgcaaa
      361 acaactgctc cgaaatggcc cgaaagaggc aagaggcatc cttggcagtc agctttcgaa
      421 acagccctaa gctgcctaaa tattcatcca ttgatgcctc attcaaagac gggccctgaa
      481 acagaacttc cagaccgctg ctcagatccg attttggagg atggtcgcca ccaccaaacg
      541 tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa
      601 tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc
      661 acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg
      721 ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca
      781 tttcaggtag cttgct
//
