LOCUS       sequence-102894-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of mCherry, EGFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ
  TITLE     PLIC proteins or ubiquilins regulate autophagy-dependent cell 
            survival during nutrient starvation.
  JOURNAL   EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 
            2009 Jan 16.
  PUBMED    19148225
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2008.238"; journalName: "EMBO Rep"; date: "2009-02"; 
            volume: "10"; issue: "2"; pages: "173-9"
COMMENT     Sequence Label: pBABE-puro mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..428
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(443..463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        448..777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      628..763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(787..806)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc cggtaccgaa ttcatgccgt
       61 cggagaagac cttcaagcag cgccgcacct tcgaacaaag agtagaagat gtccgactta
      121 ttcgagagca gcatccaacc aaaatcccgg tgataataga acgatacaag ggtgagaagc
      181 agcttcctgt tctggataaa acaaagttcc ttgtacctga ccatgtcaac atgagtgagc
      241 tcatcaagat aattagaagg cgcttacagc tcaatgctaa tcaggccttc ttcctgttgg
      301 tgaacggaca cagcatggtc agcgtctcca caccaatctc agaggtgtat gagagtgaga
      361 aagatgaaga tggattcctg tacatggtct atgcctccca ggagacgttc gggatgaaat
      421 tgtcagtgta agtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      781 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
      841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
      901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
      961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     1021 ccggagagcg tcgaagcggg g
//
