LOCUS       sequence-102896-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of mCherry, EGFP-tagged LC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ
  TITLE     PLIC proteins or ubiquilins regulate autophagy-dependent cell 
            survival during nutrient starvation.
  JOURNAL   EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 
            2009 Jan 16.
  PUBMED    19148225
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2008.238"; journalName: "EMBO Rep"; date: "2009-02"; 
            volume: "10"; issue: "2"; pages: "173-9"
COMMENT     Sequence Label: pBABE-puro mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(76..786,787..789,790..792))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(76..786,787..789,790..792))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     467..486
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     726..747
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      789..798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(807..831)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctttgttcg aaggtgcggc gctgcttgaa ggtcttctcc gacggcatga attcggtacc
       61 ggatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      121 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      181 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      241 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      301 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      361 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      421 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      481 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      541 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
      601 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      661 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
      721 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
      781 cttgctcacc atggtggcga ccggaaagcc tgcttttttg tacaaacttg ttcctgggcc
      841 agcggatctg agtccggact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
      901 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
      961 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     1021 gacctcagcg tcgta
//
