LOCUS       sequence-102898-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses hM4Dnrxn and a red fluorescent label from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AA0250-mCherry-2a-hM4D-nrxn1a rev
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..122
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    185..218
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(507..533)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(534..560)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
       61 catcattttg gcaaagaatt ggatccccat aacttcgtat aaagtatcct atacgaagtt
      121 atatcaaaat aggaagacca atgcttcacc atcgacccga attgccaagc atcaccatcg
      181 acccataact tcgtataatg tatgctatac gaagttatac tagagcctct gctaaccatg
      241 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
      301 atcattttgg caaagaattc ggtacctcga gcaccggttc acacatagta ctctttgtcc
      361 ttattcttct tgttcttatt cgctgacttc gccgatgagg gctgcttttc cttcaccacc
      421 gctccgtttg actgggcgga gttcgagata tagttccgtg actcgtccac atgatacgag
      481 ccttcgtccc ggttccgata cttgtaagcg taatccggga cgtcataggg ataggcgtaa
      541 tcggggacat catatgggta tctcgctgtc ccgatatttc ggtactggca caacaggaga
      601 tggcggaacg ttttcttgaa cgtggcgtta cacaatgcgt agcatgccgg attgatagtg
      661 gagttgacgt agcagagcca atacccgatc gaccagaccg tgtctgggat gcacgattga
      721 caaaatgtat tcaccaacac catgacatta tacggagtcc acgtcaagat aaacgccagg
      781 aggatggcga aaatcgttct tgtcaccttt ctctctctcg cggccattt
//
