LOCUS       sequence-102899-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses hM4Dnrxn and a red fluorescent label from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AA0250-mCherry-2a-hM4D-nrxn1a rev
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    170..203
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcga acctcttcga
       61 gggacataac ttcgtataat gtatgctata cgaagttatg ggtcgatggt gagatctgga
      121 ctagagggtc gatggtgatg cttggatcaa aataggaaga atcccaatga taacttcgta
      181 taaagtatcc tatacgaagt tatactagtc gagcaccggg atccaccgga agccacctgc
      241 atgcggccgc caccatggta tcaaaggggg aagaagataa catggcaatt atcaaggagt
      301 tcatgcggtt taaggtgcac atggaaggca gcgtcaacgg gcacgagttt gagatcgagg
      361 gagagggtga gggacgcccc tacgagggga cacagacagc caagctgaaa gtcaccaaag
      421 gaggaccgtt gcctttcgcg tgggacatcc tttcaccgca gtttatgtac gggtccaaag
      481 cgtatgtgaa gcatcccgcg gacattccag actatcttaa actgtcgttt cccgagggct
      541 tcaaatggga gcgagtgatg aattttgagg atggaggggt agtgacagtg acgcaagact
      601 cgtccctcca agacggcgag tttatctaca aggtgaaact cagaggcact aatttcccct
      661 cggacggtcc agtcatgcag aaaaagacga tgggttggga ggcgtcgtcg gagcggatgt
      721 atcccgaaga tggagcactc aaaggggaga tcaaacagag attgaagttg aaagacggtg
      781 gacactacga cgccgaagtc aagacaacgt ataaggcgaa gaagccggtg cagttgccgg
      841 gtgcctacaa
//
