LOCUS       sequence-102901-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of tgRFPt-Nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: tgRFPt-SSPB WT
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    105..138
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(193..522)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 ttagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
       61 tgatatcgaa ttgtacctag tggaaccgga acccttaaac atgtataact tcgtataatg
      121 tatgctatac gaagttatta ggtccctcga cgaattgcct gcaggttatc ttgatccggt
      181 ggatccttaa ccaatattca gctcgtcata gatttcttct ggttcgaaca tcacaccatc
      241 gccgttctcg cgagcgtaaa tggccagagc ggcacccatc gggatataca gttcacgaga
      301 cacgccctta aagcgggcgt tgaactggat aaaatcattt gtcagttgca ggttgccggt
      361 cgcacttgca gacagattca gcacgatctg accgtctttc acatactcca cgggcacgtt
      421 cacgcccagg tatgtggcat ccaccaccag atatggggta aagctgttat caaccagcca
      481 atcgtaatat tcacgcagca gcttagggcg tttcggggag ctgaattcct gcagggtcag
      541 gccgctcaga tcaatgccga attcgttcga agcttgagct cgagatctga gtccggactt
      601 gtacagctcg tccatgccat taagtttgtg ccccagtttg ctagggaggt cgcagtatct
      661 ggccacagcc acctcgtgct gctcgacgta ggtctctttg tcggcctcct tgattctttc
      721 cagtctgtgg tccacatagt agacgccggg catcttgagg ttcttagcgg gtttcttgga
      781 tctgtatgtg gtcttgaagt tgc
//
