LOCUS       sequence-102908-        6351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Thr412Gly-CePheRS Alpha Subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Yuet KP, Doma MK, Ngo JT, Sweredoski MJ, Graham RL, Moradian A, Hess
            S, Schuman EM, Sternberg PW, Tirrell DA
  TITLE     Cell-specific proteomic analysis in Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Feb 17. pii: 201421567.
  PUBMED    25691744
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Feb 17. pii: 201421567."
COMMENT     Sequence Label: pKPY197
FEATURES             Location/Qualifiers
     source          1..6351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1953..1979
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2028..2056)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2804..2824
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3791..3813)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3913..3932
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4010..4028)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4096..4200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4201..4269,4270..5061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4419..4438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5232..5820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5721..5740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5974..5991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6108..6129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6144..6149,6150..6167,6168..6174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6182..6198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6187..6209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6206..6222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6206..6222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgacatgaga ggatcgcatc accatcacca tcacggatcc
       61 atgaccgatc cggatcgtct caaccaaatt ctacaagatc aaccatacat tgaagggttc
      121 gtgcaaccat cggccgccga tttctctgcg agagcttcga taacagaaaa agaattgaaa
      181 gggcgagagc atttggaacg ctggtttcag catttatcat cttttgaaat gactgctgaa
      241 actgatgtcc gatcaacgga gaacctaccc caacaaatcc tcgatttcct tcaagaatcg
      301 aatgagttca actcaattca actcgcccag caatggaatc tcgatcatca gaaagtgatt
      361 ggagcaatca aaagtttgct tgcaaacgaa ggggttctta ccaccaaaga tgtgaccgag
      421 aaacggctgg aattaactaa cgagggagta cagtttgcta acgaaggttc gccggagtac
      481 ctcgtttttg agtttgttgg aactgatgga gccgcgcaag ctgacattca gaagaaacca
      541 tttggaaaaa ttggtatggc gaaagctatg cagttcaaat gggtttctgt ggataaggga
      601 cgtgtcgtcc gacaagccac tgaagtaact gattccacca gaaaacagct cgagtcgtta
      661 agaattggaa gttccgatgt tagtgaaaat gagaagaagg agctgaagaa gagaaagctt
      721 atcagtgaag taaacatcaa agcattggtg gtttccaagg gaacatcttt caccactagt
      781 cttgcaaagc aggaagctga tttgactccg gaaatgattg cttctggttc atggaaagac
      841 atgcaattca aaaagtataa tttcgattca ctcggagttg ttccgtcatc tgggcatctg
      901 catccattaa tgaaagtgcg gtctgagttc cgacaaatct tcttctcaat gggattttct
      961 gaaatggcga caaatcgata cgtggagtcg tctttctgga actttgatgc ccttttccaa
     1021 cctcaacagc atcctgcaag agatgctcat gatactttct tcgtttctga tcccgcgatt
     1081 agcacgaagt tccctgaaga ttatttggag agagtaaaaa ctgttcattc gaaaggggga
     1141 tatggttccg ctggatataa ctacgactgg aagattgaag aggctcagaa gaatgttctt
     1201 agaacccata caaccgccgt atctgctcgg cagttgtacc agctagctca ggaaggtttc
     1261 cgtccatcta aactattctc gattgaccgt gtttttcgta atgagactct cgacgctaca
     1321 catttggccg agttccatca agtagaagga gtaattgctg agaaaaatct cagtttggct
     1381 catcttatcg gaatcttcac cgagttcttc aagaaattgg gaatcaccaa tcttcgtttc
     1441 aagccaacat acaatccata tggagagccg agtatggaaa ttttcgcgta ccatcaagga
     1501 cttactaaat gggtggagat tggaaactct ggaatgttcc gccctgaaat gcttctgccg
     1561 atgggtttgc cagctgatgt gaatgtcgct ggttatggtt tgtccctgga gcgcccgaca
     1621 atgattaagt atggaattaa caacattcgt gatcttttcg gaagcaaaat cgatttgaat
     1681 gtggtctaca ataatccaat ctgtagactc gacaagtaat ctccaaaatc atcgttacat
     1741 ttttttgttt tgttattttg ttacatgtat acctccatct tgatcgttaa cacttgtttt
     1801 acttgtttcc caataaaatg ttgttgatgc tgtcatgtta gggatgtttt acttttatct
     1861 ttacttggtt ctcaaccttg ccgatatttc tattctactt taattgtaac ctttattttg
     1921 ttctttgttt aatatattat tacagggtaa tgtacccata cgacgtccca gactacgctt
     1981 cggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     2041 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     2101 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     2161 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
     2221 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
     2281 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     2341 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
     2401 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
     2461 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     2521 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     2581 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
     2641 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     2701 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     2761 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     2821 taacagctgc tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt
     2881 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
     2941 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
     3001 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     3061 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     3121 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     3181 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     3241 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     3301 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     3361 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     3421 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     3481 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     3541 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     3601 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     3661 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
     3721 cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg
     3781 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3841 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     3901 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     3961 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata
     4021 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4141 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4201 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4261 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4321 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4381 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4441 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4501 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4561 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4621 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4681 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4741 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4801 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4861 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4921 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4981 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5041 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5101 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5221 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5281 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5341 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5401 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5461 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5521 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5581 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     5641 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5701 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5761 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5821 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5881 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5941 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6001 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6061 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg
     6241 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa
     6301 tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
