LOCUS       sequence-102916-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasma membrane-targeted FRET biosensor for monitoring cAMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   DiPilato LM, Zhang J
  TITLE     The role of membrane microdomains in shaping beta2-adrenergic 
            receptor-mediated cAMP dynamics.
  JOURNAL   Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009 
            May 26.
  PUBMED    19603118
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b823243a"; 
            journalName: "Mol Biosyst"; date: "2009-08"; volume: "5"; issue: 
            "8"; pages: "832-7"
COMMENT     Sequence Label: pcDNA3-Lyn-ICUE3
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(119..121,122..124,125..835)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(119..121,122..124,125..835)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(119..121,122..124,125..835)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(164..185)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(425..444)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     772..793
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcg gccgccacca tgggctgcat caagagcaag cgcaaggaca aggatcccat
      121 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      181 cgacgtaaac ggccacaggt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      241 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      301 cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      361 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgta ccatcttctt
      421 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      481 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      541 gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
      601 catcaaggcc cacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      661 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      721 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      781 gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaaggctgg
      841 tacccc
//
