LOCUS       sequence-102928-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rac1 constitutively active mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB rac1 QL
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(75..470,471..473,474..653))
                     /codon_start=1
                     /product="constitutively active mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (Q61L)
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGLEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(690..709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(691..709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(754..957)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(783..803)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagcgg ccgctcgagt
       61 cgacccggga attcttacaa cagcaggcat tttctcttcc tcttcttcac gggaggcggg
      121 cagaggactg ctcggatcgc ttcgtcaaac actgtcttga ggcctcgctg tgtgagcgcc
      181 gagcactcca ggtattttac agcaccaatc tccttagcca tggctagacc ctgcggatag
      241 gtgatgggag tcagcttctt ctccttcagt ttctcgatcg tgtctttatc atccctaaga
      301 tcaagtttag ttcccactag gatgatggga gtgttgggac agtggtgccg cacctcagga
      361 taccactttg cacggacatt ttcaaatgat gcaggactca caagggaaaa gcaaattaag
      421 aacacatctg tttgcggata ggataggggg cgtaatctgt cataatcctc gagtccagct
      481 gtatcccata agcccagatt caccggtttt ccatctacca taacattggc agaataattg
      541 tcaaagacag tagggatata ttctccagga aatgcattgg ttgtgtaact gatcagtagg
      601 caagttttac ctacagctcc gtctcccacc accacacact tgatggcctg catggtggcg
      661 gatccgagct cggtaccaag cttgggtctc cctatagtga gtcgtattaa tttcgataag
      721 ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg
      781 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      841 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      901 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      961 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     1021 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     1081 tggcatatga
//
