LOCUS       sequence-102929-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rhoA constitutively active mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB rhoA QL
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      111..120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(117..302,303..305,306..698)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     promoter        complement(753..771)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(754..771)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(791..808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    797..908
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc gccaccatgg
      121 ctgccatccg gaagaaactg gtgattgttg gtgatggagc ctgtggaaag acatgcttgc
      181 tcatagtctt cagcaaggac cagttcccag aggtgtatgt gcccacagtg tttgagaact
      241 atgtggcaga tatcgaggtg gatggaaagc aggtagagtt ggctttgtgg gacacagctg
      301 ggctcgagga ttatgatcgc ctgaggcccc tctcctaccc agataccgat gttatactga
      361 tgtgtttttc catcgacagc cctgatagtt tagaaaacat cccagaaaag tggaccccag
      421 aagtcaagca tttctgtccc aacgtgccca tcatcctggt tgggaataag aaggatcttc
      481 ggaatgatga gcacacaagg cgggagctag ccaagatgaa gcaggagccg gtgaaacctg
      541 aagaaggcag agatatggca aacaggattg gcgcttttgg gtacatggag tgttcagcaa
      601 agaccaaaga tggagtgaga gaggtttttg aaatggctac gagagctgct ctgcaagcta
      661 gacgtgggaa gaaaaaatct ggttgccttg tcttgtgaga attcccgggt cgactcgagc
      721 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      781 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      841 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      901 attgcatcgc attgtctgag taggtgtcat tctattctgg ggg
//
