LOCUS       sequence-102931-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of rhoA constitutively active mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Coso OA, Chiariello M, Yu JC, Teramoto H, Crespo P, Xu N, Miki T, 
            Gutkind JS
  TITLE     The small GTP-binding proteins Rac1 and Cdc42 regulate the activity 
            of the JNK/SAPK signaling pathway.
  JOURNAL   Cell. 1995 Jun 30;81(7):1137-46.
  PUBMED    7600581
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1995-06-30"; volume: "81(7)"; pages: "1137-46"
COMMENT     Sequence Label: pCDNAIIIB rhoA QL
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(75..467,468..470,471..656))
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     regulatory      653..662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(693..712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(694..712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(757..960)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(786..806)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catttaggtg acactataga atagggccct ctagatgcat gctcgagcgg ccgctcgagt
       61 cgacccggga attctcacaa gacaaggcaa ccagattttt tcttcccacg tctagcttgc
      121 agagcagctc tcgtagccat ttcaaaaacc tctctcactc catctttggt ctttgctgaa
      181 cactccatgt acccaaaagc gccaatcctg tttgccatat ctctgccttc ttcaggtttc
      241 accggctcct gcttcatctt ggctagctcc cgccttgtgt gctcatcatt ccgaagatcc
      301 ttcttattcc caaccaggat gatgggcacg ttgggacaga aatgcttgac ttctggggtc
      361 cacttttctg ggatgttttc taaactatca gggctgtcga tggaaaaaca catcagtata
      421 acatcggtat ctgggtagga gaggggcctc aggcgatcat aatcctcgag cccagctgtg
      481 tcccacaaag ccaactctac ctgctttcca tccacctcga tatctgccac atagttctca
      541 aacactgtgg gcacatacac ctctgggaac tggtccttgc tgaagactat gagcaagcat
      601 gtctttccac aggctccatc accaacaatc accagtttct tccggatggc agccatggtg
      661 gcggatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat taatttcgat
      721 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      781 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      841 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      901 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      961 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     1021 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     1081 acttggcata tgatacactt gatgtactgc ca
//
