LOCUS       sequence-102945-         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 integrase F185K/C280S in pET-15b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Jenkins TM, Engelman A, Ghirlando R, Craigie R
  TITLE     A soluble active mutant of HIV-1 integrase: involvement of both the 
            core and carboxyl-terminal domains in multimerization.
  JOURNAL   J Biol Chem. 1996 Mar 29;271(13):7712-8.
  PUBMED    8631811
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-03-29"; volume: "271(13)"; pages: "7712-8"
COMMENT     Sequence Label: HIV-1 integrase F185K/C280S in pET-15b
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..67
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
       61 cggcagccat atgtttttag atggaataga taaggcccaa gaagaacatg agaaatatca
      121 cagtaattgg agagcaatgg ctagtgattt taacctgcca cctgtagtag caaaagaaat
      181 agtagccagc tgtgataaat gtcagctaaa aggggaagcc atgcatggac aagtagactg
      241 tagcccagga atatggcagc tagattgtac acatttagaa ggaaaagtta tcttggtagc
      301 agttcatgta gccagtggat atatagaagc agaagtaatt ccagcagaga cagggcaaga
      361 aacagcatac ttcctcttaa aattagcagg aagatggcca gtaaaaacag tacatacaga
      421 caatggcagc aatttcacca gtactacagt taaggccgcc tgttggtggg cggggatcaa
      481 gcaggaattt ggcattccct acaatcccca aagtcagggg gtaatagaat ctatgaataa
      541 agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta agacagcagt
      601 acaaatggca gtattcatcc ac
//
