LOCUS       sequence-102961-        9155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p158
FEATURES             Location/Qualifiers
     source          1..9155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             219..230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1287..1331
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1384..1431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1484..1508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1523..1545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2642..2686
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2739..2786
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2820..2838
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2839..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2878..2900
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4090..4134
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4187..4234
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4278..4733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4575..4596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4848..5639,5640..5708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5471..5490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5709..5801)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5882..6470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6371..6390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6624..6641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6656..6798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6724..6743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6884..6906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6900..7091)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7663..8745)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8707..8726
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8746..8823)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9029..9048
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9139..9155,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
      121 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
      181 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
      241 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
      301 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
      361 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
      421 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
      481 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
      541 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
      601 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
      661 aagtggtgat caacttctat ccgaaatgtc cgcagccgga tctgaccctg ggtctgaaac
      721 gtcataccga tccgggtaca attaccctgc tgctgcaaga tcaggtgggt ggtctgcagg
      781 caacccgtga tggtggcaaa acctggatta ccgttcagcc ggttgaaggt gcatttgttg
      841 ttaatctggg tgatcatggc cattatctga gcaatggtcg ctttaaaaac gcagatcatc
      901 aggcagttgt taatagcaat tgtagccgtc tgagcattgc aacctttcag aatccggcac
      961 cggaagcaac cgtttatccg ctgaaaattc gtgaaggtga aaaaccgatt ctggaagaac
     1021 cgattacctt tgccgatatg tataaacgca aaatgagcaa agatatcgag ctggccaaac
     1081 tgaaaaaact ggcgaaagaa aaaaaactgc tgcaagacca gcaggatatc gaaaaagcaa
     1141 aactggaaat caaaagcacc gatgaaatct tcgccctggt tggtgcactg atgcatgtta
     1201 tgcagaaacg tagccgtgca attcatagca gtgatgaagg tgaagatcaa gccggtgatg
     1261 aagatgagga ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
     1321 acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa
     1381 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctaggatcg
     1441 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     1501 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgaaaga
     1561 tagcgttgca agcgcaaccg caagcgcacc gggtacagtt tgtgttaccg gtgcagcagg
     1621 ttttattggt agctggctgg ttatgcgtct gctggaacgt ggttatattg ttcgtgcaac
     1681 cgttcgtgat ccggcaaatc tgaaaaaagt taaacatctg ctggatctgc cgaaagcaga
     1741 taccaatctg accctgtgga aagccgatct gaatgaagag ggtagctttg atgaagcaat
     1801 tgaaggttgt agcggtgttt ttcatgttgc aaccccgatg gattttgaaa gcaaagatcc
     1861 ggaaaacgaa gtgattaaac cgaccattaa cggtgtgctg agcattattc gtagctgtac
     1921 caaagcaaaa accgttaaac gtctggtttt taccagcagc gcaggtacag ttaatgttca
     1981 agaacatcag cagccggtgt ttgatgaaaa caattggagc gatctgcact tcatcaacaa
     2041 aaaaaaaatg accggctgga tgtattttgt gagcaaaacc ctggcagaaa aagcagcatg
     2101 ggaagcagca aaagaaaaca acattgattt catcagcatt atcccgaccc tggttggtgg
     2161 tccgtttatt atgccgacct ttccgcctag cctgattacc gcactgagcc cgattacccg
     2221 taatgaaggt cattattcca ttatcaaaca gggccagttt gtgcatctgg atgatctgtg
     2281 tgaaagccac atttttctgt atgaacgtcc gcaggcagaa ggtcgttata tttgtagcag
     2341 ccatgatgca accattcatg atctggccaa actgatgcgt gaaaaatggc ctgaatataa
     2401 tgttccgacc gaattcaaag gcatcgataa agatctgccg gttgttagct tttccagcaa
     2461 aaaactgatt ggcatgggct tcgagttcaa atatagcctg gaagatatgt ttcgtggtgc
     2521 cattgatacc tgtcgtgaaa aaggtctgct gccgcatagc tttgcagaaa atccggttaa
     2581 tggcaacaaa gtgcctcctc ctgcactgcc tccgaaacgt cgtcgttaac tcgagtctgg
     2641 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     2701 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     2761 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     2821 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     2881 gtttaacttt aagaaggaga tatacatatg accgttagcg gtgcaattcc gagcatgacc
     2941 aaaaatcgta ccctggttgt tggtggcacc ggttttattg gtcagtttat taccaaagca
     3001 agcctgggtt ttggttatcc gacctttctg ctggttcgtc cgggtccggt tagcccgagc
     3061 aaagcagtta ttatcaaaac ctttcaggat aaaggtgcca aagtgattta tggcgtgatc
     3121 aacgataaag aatgcatgga aaaaattctg aaagagtacg agatcgacgt tgttattagc
     3181 ctggtgggtg gtgcacgtct gctggatcag ctgaccctgc tggaagcaat taaaagcgtt
     3241 aaaaccatca aacgttttct gccgagcgaa tttggccatg atgttgatcg taccgatccg
     3301 gttgaaccgg gtctgaccat gtataaagaa aaacgtctgg tgcgtcgtgc cgttgaagaa
     3361 tatggtattc cgtttaccaa tatctgctgc aatagcattg caagctggcc gtattatgat
     3421 aattgtcatc cgagccaggt tccgcctccg atggatcagt ttcagattta tggtgatggt
     3481 aacaccaaag cctatttcat tgatggcaac gatatcggca aatttaccat gaaaaccatc
     3541 gatgatattc gcaccctgaa caaaaatgtt cattttcgtc cgagcagcaa ctgctacagc
     3601 attaatgaac tggcaagcct gtgggagaaa aaaatcggtc gtacactgcc tcgttttacc
     3661 gttaccgcag ataaactgct ggcacatgca gcagaaaaca ttattccgga aagcattgtt
     3721 agcagcttta cccacgatat ctttattaac ggttgccagg tgaactttag catcgatgaa
     3781 catagtgatg tggaaatcga tacactgtat ccggatgaaa aatttcgtag cctggatgat
     3841 tgctatgaag attttgttcc gatggtgcac gataaaattc atgcaggtaa aagcggtgaa
     3901 atcaaaatca aagatggtaa accgctggtt cagaccggca ccattgaaga aattaacaaa
     3961 gacattaaaa ccctggtgga aacccagccg aatgaagaga tcaaaaaaga tatgaaagca
     4021 ctggttgaag ccgttccgat tagcgcaatg ggtggtgtta aagaaagcct ggtttaactc
     4081 gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact
     4141 agtcgcagct taattaacct aaactgctgc caccgctgag caataactag cataacccct
     4201 tggggcctct aaacgggtct tgaggggttt tttgctagcg aaaggaggag tcgactatat
     4261 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4321 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4381 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4441 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4501 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4561 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4621 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4681 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttctggcgg
     4741 cacgatggca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5701 atactcatac tcttcctttt tcaatcatga ttgaagcatt tatcagggtt attgtctcat
     5761 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggtcatg accaaaatcc
     5821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6001 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     6661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     6721 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     6781 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6841 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6901 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     6961 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     7021 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     7081 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     7141 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     7201 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     7261 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     7321 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     7381 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     7441 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     7501 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgct agtcatgccc
     7561 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     7621 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     7681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7741 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     7801 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     7861 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     7921 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     7981 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     8041 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     8101 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     8161 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     8221 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     8281 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     8341 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     8401 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     8461 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     8521 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     8581 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     8641 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     8701 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     8761 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     8821 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     8881 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     8941 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     9001 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     9061 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagcctagg atcgagatcg
     9121 atctcgatcc cgcgaaatta atacgactca ctata
//
