LOCUS       sequence-102963-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of ITSN DH/PH-tgRFPt-Nano.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..413)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattgtacc tagtggaacc
       61 ggaaccctta aacatgttta accaatattc agctcgtcat agatttcttc tggttcgaac
      121 atcacaccat cgccgttctc gcgagcgtaa atggccagag cggcacccat cgggatatac
      181 agttcacgag acacgccctt aaagcgggcg ttgaactgga taaaatcatt tgtcagttgc
      241 aggttgccgg tcgcacttgc agacagattc agcacgatct gaccgtcttt cacatactcc
      301 acgggcacgt tcacgcccag gtatgtggca tccaccacca gatatggggt aaagctgtta
      361 tcaaccagcc aatcgtaata ttcacgcagc agcttagggc gtttcgggga gctgaattcc
      421 tgcagggtca ggccgctcag atcaatgccg aattcgttcg aagcttgagc tcgagatctg
      481 agtccggact tgtacagctc gtccatgcca ttaagtttgt gccccagttt gctagggagg
      541 tcgcagtatc tggccacagc cacctcgtgc tgctcgacgt aggtctcttt gtcggcctcc
      601 ttgattcttt ccagtctgtg gtccacatag tagacgccgg gcatcttgag gttcttagcg
      661 ggtttcttgg atctgtatgt ggtcttgaag ttgcagatca ggtggccccc gcccacgagc
      721 ttcagggcca tgtcggttct gccttccagg ccgccgtcag cggggtacag catctcggtg
      781 ttggcctccc agccgagtgt tttcttctgc atcaca
//
