LOCUS       sequence-102968-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty sgRNA expression vector for expression of sgRNA for Sp Cas9
            (3rd generation lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Pham H, Kearns NA, Maehr R
  TITLE     Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian 
            Cells.
  JOURNAL   Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3.
  PUBMED    26463376
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1358:43-57. doi"; pages: " 
            10.1007/978-1-4939-3067-8_3"
COMMENT     Sequence Label: pLenti SpBsmBI sgRNA Puro
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(662..682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccatgaccga gtacaagcct actgttcggc tcgcgactcg cgatgatgtc cccagagcag
       61 tgagaacact ggctgctgcc tttgccgatt accccgcaac ccgccatacc gtggatcccg
      121 accggcacat cgagagggtg actgaactgc aggaattgtt tttgacaagg gtgggactcg
      181 acattggaaa ggtgtgggtg gccgatgacg gagccgccgt cgccgtttgg accacaccag
      241 agagcgtcga agccggcgcg gtgttcgccg agatcggccc aagaatggcc gagctctctg
      301 gatctagact ggccgcgcaa cagcagatgg aaggcctgct cgcgccacac cgacctaagg
      361 agcccgcatg gttcctggcc accgtaggcg tttcccccga tcaccaggga aaggggcttg
      421 gcagcgccgt agttctgccc ggcgtggagg ccgctgaacg agctggtgtt cccgcatttc
      481 ttgagaccag tgcacctagg aaccttcctt tctatgaaag gttgggtttc accgtcaccg
      541 ccgatgtgga agtgcctgag ggacctcgaa cctggtgcat gactcgaaag cctggcgcct
      601 aactcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
      661 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
      721 ct
//
