LOCUS       sequence-102973-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of ITSN DH/PH-tgRFPt-Micro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear 
            JE, Kuhlman B
  TITLE     Engineering an improved light-induced dimer (iLID) for controlling 
            the localization and activity of signaling proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 
            10.1073/pnas.1417910112. Epub 2014 Dec 22.
  PUBMED    25535392
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1417910112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-01-6- 6"; volume: "112"; issue: "1"; pages: "112-7"
COMMENT     Sequence Label: pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(86..205,206..208,209..415))
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgtt taaccaatat tcagctcgtc atagatttct tctggttcga
      121 acatcacacc atcgccgttc tcgcgagcgt aaatggccag agcggcaccc atcgggatat
      181 acagttcacg agacacgccc ttaaactggg cgttgaactg gataaaatca tttgtcagtt
      241 gcaggttgcc ggtcgcactt gcagacagat tcagcacgat ctgaccgtct ttcacatact
      301 ccacgggcac gttcacgccc aggtatgtgg catccaccac cagatatggg gtaaagctgt
      361 tatcaaccag ccaatcgtaa tattcacgca gcagcttagg gcgtttcggg gagctgaatt
      421 cctgcagggt caggccgctc agatcaatgc cgaattcgtt cgaagcttga gctcgagatc
      481 tgagtccgga cttgtacagc tcgtccatgc cattaagttt gtgccccagt ttgctaggga
      541 ggtcgcagta tctggccaca gccacctcgt gctgctcgac gtaggtctct ttgtcggcct
      601 ccttgattct ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag
      661 cgggtttctt ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga
      721 gcttcagggc catgtcggtt ctgccttcca ggccgccgtc agcggggtac agcatctcgg
      781 tgttggcctc cca
//
