LOCUS       sequence-102974-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating full length gene 60 mRNA by in vitro 
            transcription with T7 RNA pol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(638..656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(690..707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(690..706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(699..721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcttttgcc caattaatta taccacatcc ttgtggtaaa gtaaactact ggctcatcca
       61 ttctttacga aggtcagcat tatctcccat gagcatttca aaaagctctt tccagttctc
      121 aggaagttta acaacatcat atactgggtt ttgaatcatc tcacgatatt cagatttttc
      181 caaagagcca agtcccttaa tataacggat gctatgttta ggtagagcat ctttggcact
      241 ctcatattca gcgactgtat aaaaccattc ttgtttttta ccgacctgag cgatgattac
      301 aggagttttg acaaagcgaa ttcgtccttg ctcaaacaat tctggccaat tactaaaaaa
      361 tccgagcaga gaaggataaa tagaacctaa tccaataatc tcttaattat gaggtatttc
      421 tatagatagc ccgaaggcta tccatcgtga tctgcgtctg tcataatagc gacattcgca
      481 tagttcattg aagaggattt aatagaacga cgaacattgt tctgcaattt atattttttc
      541 atatcaacgc tagaagaatc aatttttaca aatttcatta tacacctcat agaacttttc
      601 atcaggaatc caaccgcgtt taaattcatt aaatgctcct atagtgagtc gtattatcta
      661 gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
      721 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      781 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      841 gaatggcgcc tgatgcggta tt
//
