LOCUS       sequence-102977-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA, sequence 5' of coding gap + 
            Cys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 to gap + Cys
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(249..267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(301..318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(301..317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(310..332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(526..545)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     645..667
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttttgcc caattaatta taccacatcc ttgtggtaaa gtaaactaac atccatcgtg
       61 atctgcgtct gtcataatag cgacattcgc atagttcatt gaagaggatt taatagaacg
      121 acgaacattg ttctgcaatt tatatttttt catatcaacg ctagaagaat caatttttac
      181 aaatttcatt atacacctca tagaactttt catcaggaat ccaaccgcgt ttaaattcat
      241 taaatgctcc tatagtgagt cgtattatct agaggatccc cgggtaccga gctcgaattc
      301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      421 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      481 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      541 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      601 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      661 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 ggg
//
