LOCUS       sequence-102978-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for generating gene 60 mRNA truncated two codons after 
            coding gap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Todd GC, Walter NG
  TITLE     Secondary structure of bacteriophage T4 gene 60 mRNA: implications 
            for translational bypassing.
  JOURNAL   RNA. 2013 May;19(5):685-700. doi: 10.1261/rna.037291.112. Epub 2013 
            Mar 14.
  PUBMED    23492219
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037291.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "685-700"
COMMENT     Sequence Label: pUC19 gene 60 thru gap
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(302..320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(354..370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(363..385)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(579..598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     698..720
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(758..776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttttgcc caattaatta taccacatcc ttgtggtaaa gtaaactaac ctaatccaat
       61 aatctcttaa ttatgaggta tttctataga tagcccgaag gctatccatc gtgatctgcg
      121 tctgtcataa tagcgacatt cgcatagttc attgaagagg atttaataga acgacgaaca
      181 ttgttctgca atttatattt tttcatatca acgctagaag aatcaatttt tacaaatttc
      241 attatacacc tcatagaact tttcatcagg aatccaaccg cgtttaaatt cattaaatgc
      301 tcctatagtg agtcgtatta tctagaggat ccccgggtac cgagctcgaa ttcactggcc
      361 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      421 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      481 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      541 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      601 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      661 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      721 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      781 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      841 gtgcgcggaa cccctatttg tttattt
//
